While the mechanisms driving activation of the innate immune response during viral infection are well understood, the spatial organization remains poorly defined. Using in vitro models of the respiratory tract, we aim to dissect how spatial structure shapes the activation of the innate immune response during influenza A virus infection.
Axioscan image of human bronchial epithelial cells (HBECs). Stained with DAPI (blue) and Phalloidin (green)
Joel Rivera Cardona from the Brooke Lab
This is an image of a gelatin methacrylate model for the perivascular niche of the bone marrow. The perivascular niche, defined by proximity to arteriolar and sinusoidal vessels that comprise endothelial and mesenchymal cells within the bone marrow, regulates hematopoietic stem cell proliferation, differentiation, and quiescence through secreted factors, cell-cell interactions, and local remodeling of the bone marrow.
In this image, we have Hoechst 33342-stained nuclei in blue, murine bone marrow-derived mesenchymal stem cells (mMSCs) stained for podoplanin in cyan, and murine bone marrow-derived endothelial cells (mBMECs) stained for CD31 in green.
Gunnar Thompson from the Harley Lab
Two-photon image deep into the whole brain of a mouse fetus (Gestational Day 16.5) and post-processed with the Spectral Unmixing Module. Shown is the cerebellum structure and labels are for: CD31 (Green), Claudin-5 (red), Hoechst (Blue). The fetus was cleared with an adapted form of a new tissue clearing technique called ADAPT-3D.
Image taken with LSM 980
Image Courtesy: Fernando Rigal from the Antonson Lab
3D reconstruction of Salmonella Typhimurium biofilms in presence of differentially charged micro-nanoplastics (MNP). The study examined how MNP exposure influences bacterial attachment, aggregation, and extracellular polymeric substance (EPS) production- key factors that enhance biofilm resilience in food processing environments. Confocal laser scanning microscopy (CLSM) was used to visualize biofilm architecture and development over time. Understanding these interactions is critical, as enhanced biofilm formation may increase bacterial persistence, antimicrobial resistance, and the risk of contamination, posing serious threats to public health.
LSM 880, stained with FilmTracerFM 1-43 green biofilm cell stain
From Jayita De in the Pratik Banerjee Lab
An unexpected discovery of a bird skeleton within a nest built in a wooden enclosure provides new insights into nesting behavior and material composition. This finding informs the ongoing design of a concrete nest, exploring the intersection of natural instincts and architectural innovation.
Author: Arpit Amarseda
"This SBF-SEM image displays the cell body of a CA1 pyramidal neuron in the hippocampus. Our study aims to investigate mitochondrial morphology and complexity in the mouse hippocampal neurons."
Image taken on Zeiss Sigma VP SBF-SEM
Authors: Kingsley Boateng (IGB Core) and Vipendra Kumar (Nien-Pei Tsai’s
Lab).
"Ex vivo coronal section of glioma cells, labeled with GFP (green), in a mouse model of glioblastoma (GBM) on day 14 of tumor development."
Image taken on Axioscan.
Author: Urbi Saha - Andrew Smith's Lab
Cryptosporidium parvum is a protozoan parasite with a single host lifecycle composed of asexual (trophozoites and meronts) and sexual (female and male gamonts) replicative stages. This parasite is microscopic in size (~2-6 µm) when compared to its host cells (~25µm). To visualize the developmental stages of the parasite in higher resolution we labeled parasite proteins and utilized antibodies, stains, and SR-SIM. In the leftmost image there are two meronts (in green parasite protein, red parasite stain, blue nuclear stain), on top right corner the merozoites are escaping from the vacuole and on the bottom, there is a mature meront. The center image shows two male gamonts (in red parasite protein, green parasite stain, blue nuclear stain) and two female gamonts (green parasite stain) next to each other. The rightmost image shows mature male gametes escaping from the gamont in an event refer to as “egress”.
Author: Maria Nava - Sumiti Vinayak Lab.
“The tissue is from a mouse uterus that was stained with a Ki-67 antibody. Ki-67 is a nuclear antigen that is expressed in cells that are in G1, S, G2, and mitosis, but not G0 marking the process of cell proliferation.”
Author: Adriana Andrus - Romana Nowak Lab
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