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Widefield Fluorescence Microscope

Widefield Fluorescence Microscope

Widefield microscopy for fluorescence and transmitted light applications. Phase contrast, DIC, and FRET, and fluorescent filter options are available. An incubation chamber with CO2 with temperature controls is available for live cell imaging.

System Configuration

Light source: Halogen lamp for bright-field microscopy, mercury lamp (X-Cite series 120 PC)

Fluorescence Filter Cubes:  DAPI, GFP, CY3, CY5

FRET Filters: CFP, YFP

Objectives: 10x/0.3 Dry, 20x/0.8 Dry, 40X/0.6 Dry, 40x/1.2 Water, 63x/1.4 Oil, 63 X/1.4 oil (Ph3)

Cameras: Axiocam 506 color, Axiocam 506 b&w, and Orca Flash 4.0 v3 CMOS

Acquisition software: Zen Black (Zen 2.3)

 

Manufacturer: Zeiss

Equipment Model: Zeiss Axiovert 200M

Location: 121C, Carl R. Woese Institute for Genomic Biology

Contact Person: Austin Cyphersmith, 217-244-9654, ajcypher@igb.illinois.edu

FRET Information: https://www.microscopyu.com/applications/fret/basics-of-fret-microscopy

Köhler Illumination: https://www-app.igb.illinois.edu/core-docs/books/igb-core-training/page/kohler-illumination

Training Materials:

Axiovision Take Off Guide

Instructions for Advanced Techniques

Quick Reference Guide(pdf version)